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Epigenetic modifications affecting long-term gene expression DNA methylation changes: GHK-Cu may affect DNA methyltransferase activity altering cytosine methylation patterns

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Firstly, basal media composed of Advanced RPMI (Gibco) and 1% GlutaMax (Gibco) was supplemented with CHIR99021 (Tocris) and 5ng/mL recombinant human noggin (Peprotech), was added to hiPSCs following the 24 h DMSO treatment previously described

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References Demir S, Nawroth PP, Herzig S, Ekim Ustunel B